Core Facilities laboratories, utilizing the latest analytical technologies, are currently focused on cell imaging, flow cytometry, genetic sequencing, histology, metabolic functions, and animal imaging. Additionally, we offer a bioinformatics capability to support research design, implementation, and data analysis. The laboratories are available to the many pediatric basic research teams based at the John G. Rangos Sr. Research Center and are also available to other researchers from beyond the hospital system. Information about the labs, including contacts are provided below.
Cell Imaging
Equipped with confocal microscopes and devices for live cell imaging and laser capture microdissection.
Cell Imaging Core Laboratory
5th Floor, Rangos Research Center
Director: Krishna Prasadan, PhD
Contact: Dr. Prasadan, krishna.prasadan@chp.edu
412-692-9211
Cell Imaging Facility Services and Usage Guidelines (PDF)
Flow Cytometry
Analytical flow cytometry and sterile cell sorting equipment and services.
Flow Cytometry Core Laboratory
8th Floor and 6th Floor, Rangos Research Center
Director: Abbe deVallejo, PhD
Contact: Joshua Michel, joshua.michel@chp.edu
8th Floor: 412-692-6968
Flow Cytometry Facility Services and Usage Guidelines (PDF)
Genetic Sequencing
Offering Illumina next-generation sequencing services, including DNA and RNA extraction, sequencing and up-front services.
Health Sciences Sequencing Core
8th Floor, Rangos Research Center, Room 8145
Director: Amanda Poholek, PhD
Contact: Will MacDonald, w.a.macdonald@pitt.edu
412-692-3555
Get more information about this core facility.
Guide to Next Generation Sequencing
Metabolic Core
Analytical services to support research in energy metabolism, metabolic pathways, and cellular metabolic function including mass spectrometry (LC/MS/MS), Seahorse® analyzer, amino acid analyzer, and reverse-phase HPLC system.
Metabolic Core Laboratory
5th Floor, Rangos Research Center, Room 5156
Director: Clinton Van’t Land, PhD
Contact: Dr. Van’t Land, clinton.vantland@chp.edu
412-692-7652
Animal Imaging
Including “micro” magnetic resonance imaging (MRI) and Computer Tomography (CT) systems, high-resolution ultrasound and bioluminescence systems.
Animal Imaging Core Laboratory
4th Floor, Rangos Research Center
Director: Yijen Wu, PhD
Contact: Dr. Wu, yijen.wu@chp.edu
412-692-9902
Animal Imaging Facility Services and Usage Guidelines (PDF)
Bioinformatics
Study design implementation support, including high-throughput data analysis, statistical support, proposal writing, and grantsmanship, as well as faculty collaborations.
Bioinformatics Core Facility
8132 Rangos Research Center
Director: Dhivyaa Rajasundaram, PhD
Contact: Dr. Rajasundaram, dhr11@pitt.edu
412-692-8120
